Dis Markers ; Mineral bone disorders MBDs constitute a hallmark of CKD, and human papillomavirus replication process cardiovascular complications, they underlie a poor prognosis for these patients. Thus, our study focused on novel CKD biomarker patterns and their impact on the clinical staging of the disease.
As a first testing approach, the relative expression levels of proteins were assessed by the Proteome Profiler Cytokine Array Kit for pooled CKD stage serum samples to establish an overall view regarding the human papillomavirus replication process involved in CKD human papillomavirus replication process. Among the molecules that displayed significant dysregulation in the CKD stages, we further explored the involvement of Dickkopf-related protein 1 Dkk-1a recognised inhibitor of the Wnt human papillomavirus replication process pathway, and its crosstalk with 1,25OH2D3 calcitriol as new players in renal bone and vascular disease.
The serum levels of these two molecules were quantified by an ELISA 76 samplesand the results reveal decreasing circulating levels of Dkk-1 and calcitriol in advanced CKD stages, with their circulating expression showing a downward trend as the CKD develops.
Our results show that the molecules involved in orchestrating the human papillomavirus replication process response, interleukin-6 IL-6 and tumour necrosis factor alpha TNFαas well as the mineral biomarkers human papillomavirus replication process OPGosteocalcin OCosteopontin OPNand fibroblast growth factor 23 FGFcorrelate with Dkk-1 and calcitriol, raising the possibility of them being potential useful CKD biomarkers.
These results reveal the impact of different biomarker patterns in CKD staging and severity, thus opening up novel approaches to be explored in CKD clinical management.